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1. The Qiaex II Gel Extraction Kit is intended for molecular biology applications

Qiaex

2. The Qiaex II Gel Extraction Kit is intended for molecular biology applications

Qiaex

3. Qiagen Qiaex II Gel Extraction Kit, 500 Extractions, 20L Elution Volume, 5g/10L Binding Capacity, DNA Sample, Silica Technology, Manual Processing, Tube Format, 40 bp to 50 kb Fragment, For Purification of DNA Fragments from Gels and Solutions, Ideal for Restriction Digestion, Labeling, Ligation, PCR, Includes 5 x 1mL Qiaex II Suspension, Buffers

Qiagen, Qiaex

4. Kit Content: Qiagen Qiaex II Gel Extraction Kit, 150 Extractions, 20L Elution Volume, 5g/10L Binding Capacity, DNA Sample, Tube Format, Silica Technology, Manual Processing, 40 bp to 50 kb Fragment, Ideal for Restriction Digestion, Labeling, Ligation, PCR, For Purification of DNA Fragments

Qiagen, Qiaex

5. 12 Qiaex II Handbook 02/99 Protocol Qiaex II Agarose Gel Extraction Protocol This protocol is designed for the extraction of 40-bp to 50-kb DNA fragments from 0.3–2% standard or low-melt agarose gels in TAE or TBE buffers

Qiaex

6. Kit contents: Qiagen Qiaex II Gel Extraction Kit, 150 Extractions, 20L Elution Volume, 5g/10L Binding Capacity, DNA Sample, Tube Format, Silica Technology, Manual Processing, 40 bp to 50 kb Fragment, Ideal for Restriction Digestion, Labeling, Ligation, PCR, For Purification of DNA Fragments from Gels and Solutions, Includes 3 x 0.5mL Qiaex II Suspension, Buffers.

Qiagen, Qiaex

7. Obtained PCR products were purified using the Qiaex® II Gel Extraction Kit from Qiagen and cloned into the pJET1.2/blunt cloning vector using the CloneJET PCR Cloning Kit from Thermo following the instructions of the manufacturers

Qiaex, Qiagen

8. Qiaex II Suspension is a suspension of silica particles to which DNA fragments bind in the presence of chaotropic salts

Qiaex

9. Qiaex II Suspension is added to solutions or solubilized agarose gel slices and binds DNA

Qiaex

10. I think this refers to the amount of DNA present in the gel slice, because the silica particles (QiaexII) have a limited binding capacity of 5 µg DNA per 10 µl QiaexII.

Qiaexii

11. I use Qiaex II Gel extraction kit

Qiaex

12. The Qiaex II Gel Purification Kit can be used for extraction and purification of DNA from either TAE or TBE agarose or polyacrylamide gels

Qiaex

13. Qiaex II Suspension : Sodium Perchlorate @ 50-100%

Qiaex

14. CTAB-extracted DNA was also purified using the Zymo (DNA Clean & Concentrator 25 kit), Qtip 100 (Qiagen Genomic-Tip 100/G), and Qiaex II Gel Extraction Kit

Qtip, Qiagen, Qiaex

15. Geneclean ~ (Bio 101, La Jolla, CA) glass beads; or Qiaex silica gel suspension (Qiagen Inc., Chatsworth, CA) according to manufacturers' instructions

Qiaex, Qiagen

16. The Qiaex II Gel Extraction Kit provides a suspension of silica particles to which DNA fragments bind in the presence of chaotropic salts.Qiaex II Suspension is added to solutions or solubilized agarose gel slices and binds DNA

Qiaex

17. The Qiaex II Gel Extraction Kit provides a

Qiaex

18. PWhere-RPE65-CFB was digested with PacI, and the RPE65-CFB transgene construct flanked by H19 insulators was isolated by agarose gel electrophoresis using the Qiaex gel extraction kit (Qiagen

Qiaex, Qiagen

19. After electrophoresis in 1.2% agarose gel, multiple bands were observed, and the brightest band of expected size was excised from the gel and purified using a Qiaex

Qiaex

20. DNA is obtained upon proteinase K lysis and purified using the Qiaex II gel extraction beads (Qiagen)

Qiaex, Qiagen

21. Qiaex II Gel Extraction Kit (150) Buffer PE : US English : United States of America : Qiaex II Gel Extraction Kit (150) Buffer QX1 + Indikator : US English : United States of America : Qiaex II Gel Extraction Kit (150) Qiaex II-Suspension : US English : United States of America

Qiaex

22. The Qiaex II gel extraction kit ® + Cetyltrimethylammoniumbromid (CTAB) was used for method 2

Qiaex

23. The Qiaex principle is based on a commercial DNA purification kit with CTAB-modification supplied by Qiagen (Hilden, Germany); preparations were performed according to the manufacturer's instructions and as described by Kuipers and colleagues

Qiaex, Qiagen

24. While there is no column in the Qiaex II, it is a bit inconvient, however, it could be scale up or down as you desire

Qiaex

25. We constructed pMitoTimer by inserting the BamHI-NotI fragments of pTimer-1 (Clontech) into pDsRed2-Mito (Clontech) vector digested with BamHI and NotI using T4 ligase (New England Biolabs) following DNA isolation by using the Qiaex II gel extraction kit (Qiagen).

Qiaex, Qiagen

26. Resuspend Qiaex II by vortexing for 30 seconds

Qiaex

27. Add 10ml Qiaex II to the sample (for £2mg DNA)

Qiaex

28. Mix by vortexing every 2 minutes to keep Qiaex II in suspension.

Qiaex

29. Qiaex II Suspension : Qiaex II-Suspension : US English : United States of America

Qiaex

30. Reports found: 1 : SDS : Product: Product component: Validity area : Qiaex II Suspension : Qiaex II-Suspension : US English : United States of America

Qiaex

31. Qiaex II Gel Extraction Kit (Scalable) QIAquick Gel Extraction (50) QIAquick Gel Extraction (250) NA1111: GenElute™ Gel Extraction Kit (70) Cleanup: 28104: QIAquick PCR Purification (50) NA1020 EC600 EC700: GenElute™ PCR Clean-Up Kit (70) GenElute™-E Single Spin DNA Cleanup Kit GenElute™-E Organic Solvent DNA Cleanup Kit: 28106

Qiaex, Qiaquick

32. The appropriate band is excised and DNA purification performed using the Qiagen Qiaquick Gel Extraction Kit (for DNA fragments <10 kb; for larger fragments, use the Qiaex Gel Extraction Kit)

Qiagen, Qiaquick, Qiaex

33. Cut gel bands, store 4C or proceed with Qiaex II purification Qiaex II purify – ELUTE with transgene injection buffer

Qiaex

34. Briefly, amplified products were subjected to agarose electrophoresis and were stained and visualized with ethidium bromide; DNA bands were then excised and extracted by using the Qiaex II kit (QIAGEN, Inc., Chatsworth, CA)

Qiaex, Qiagen

35. Vector DNA purified by cesium chloride/ethidium bromide density gradient centrifugation or anion exchange chromatography (eg, Qiaex resin, QIAGEN Inc) will be sufficiently pure for co-transfection.

Qiaex, Qiagen

36. Use Qiaex II gel extraction kit to gel purify the DNA fragment corresponding to the gene of interest with transposon insertions (see Note 20)

Qiaex

37. Alternatively, you may use the Qiagen Qiaex II gel extraction kit for fragments >50 bps

Qiagen, Qiaex

38. The pool of barcoded vectors was then linearized with BstZ17I (NEB), gel purified (Qiagen Qiaex II Gel Extraction Kit), and subjected to a Gateway LR recombination reaction (Thermo Fisher Scientific) using entry clones from the Ultimate ORF collection assembled into six pools containing approximately 3000 ORFs each

Qiagen, Qiaex

39. Fragments of ∼1 kb were purified from 0.8% preparative agarose gels using Qiaex II gel extraction kit (QIAGEN, Chatsworth, CA)

Qiaex, Qiagen

40. 20021 Qiaex ii gel extraction kit (150) 201203 taq dna polymerase (250 u) 203603 hotstartaq plus dna polymerase (250) 203605 hotstartaq plus dna polymerase (1000) 203743 qiagen fast cycling pcr kit (200) 204054 quantifast sybr green pcr kit (400) 204143 quantitect sybr green pcr kit (200) 204343 quantitect probe pcr kit (200)

Qiaex, Qiagen, Quantifast, Quantitect

41. My experience was that none of them were as efficient as the commercial kits (eg Qiagen Qiaex II or Promega Wizard kits), but they may be a starting point for further optimization.

Qiagen, Qiaex

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