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1. Escherichia coli lac repressor (LacI) is a paradigmatic transcriptional factor that controls the expression of Laczya in the lac operon

Lac, Laci, Laczya

2. Diazoefficiens DNA from scoI downstream region (comprising blr1132') and Laczya and is published in Mol Plant Microbe Interact

Laczya

3. The structural genes Laczya code for the proteins that ultimately allow E coli to transport and metabolize lactose

Laczya, Lactose

4. Transcription is not completely eliminated, but Laczya mRNA is transcribed only at very low levels

Laczya, Low, Levels

5. PRJPaph-Laczya Sequences (7) Addgene Sequences: Partial (6) Depositing Scientist Sequences: Full (1) Full Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results

Laczya, Laboratory

6. F– φ80lacZΔ M15 Δ (Laczya-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1 For Research Use Only

Laczya

7. F– φ80lacZΔ M15 Δ (Laczya-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1 For Research Use Only

Laczya

8. Structural genes: Laczya lacZ encodes b-galactosidase, which cleaves the disccharide lactose into galactose and glucose

Laczya, Lacz, Lactose

9. F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(Laczya-argF)U169, hsdR17(rK- mK+), λ– F-: This strain does not carry the F plasmid (DNA plasmid called Fertility Factor or Sex Factor)

Laczya

10. F–, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, Φ80d lacZΔ M15, Δ (Laczya - argF) U169, Δ (mrr - hsdRMS - mcrBC), ΔmcrA, λ– Please Read Before Proceeding with Transformation Use no more competent cells than necessary

Lacz, Laczya

11. Characterization of stress-responsive behavior in Pseudomonas aeruginosa PAO: isolation of Tn3-Laczya fusions with novel damage-inducible (din) promoters

Laczya

12. If Laczya mRNA is translated, and the inducer is removed after, what happens to the levels of Laczya proteins

Laczya, Levels

13. Cyclic adenosine 3',5'-monophosphate-mediated hyperinduction of araBAD and Laczya expression in a crp mutant of Escherichia coli K-12

Laczya

14. Transferred via homologous recombination to the complete lac operon (Laczya) on the F' episome

Lac, Laczya

15. A) express Laczya constitutively

Laczya

16. B) not induce Laczya in the presence of lactose

Laczya, Lactose

17. Xpression of the three gene fusions studied, dinA::Laczya, dinB::Laczya, and dinC::Laczya, increased following UVC and quinolone

Laczya

18. Higher levels of beta-galactosidase were produced by ptxA-Laczya in the presence of bvgAS during growth in Stainer-Scholte medium or M9 minimal salts medium with glucose than in Luria-Bertani medium

Levels, Laczya, Luria

19. In contrast, the level of fhaB-Laczya expression was high during growth in all media.

Level, Laczya

20. The Laczya operon houses genes encoding proteins needed for lactose breakdown

Laczya, Lactose

21. The gene lacI is situated immediately upstream of Laczya but is transcribed from a lacI promoter

Laci, Laczya

22. Laczya fusions were grown in L broth to midlogarithmic phase as described (11)

Laczya

23. Construction of bvg: :Laczya Fusions

Laczya

24. Restriction fragments containing the 5' region of the bvgABC operon were isolated and inserted upstream of the promoterless Laczya genes in the plasmid pRS551 (16).

Laczya

25. The pseudo-operator sequence O 2 is located within the lacZ gene, and the primary operator sequence O 1 overlaps the promoter sequence for the Laczya metabolic genes (Figure 1)

Located, Lacz, Laczya

26. The principal open problem is that of determining the mechanisms of repression and activation of the Laczya genes and the role of the auxiliary operators in those mechanism

Laczya

27. From this position RNAP proceeds to transcribe all three genes (Laczya) into mRNA

Laczya

28. Coli lac operon, the Laczya mRNA, and the lacI genes are available from GenBank (view)

Lac, Laczya, Laci

29. NA NA •RNA ON OFF Determine the nature of the expression of the Laczya genes, represented by L, and label cach genotype as constitutive, inducible, or no transcription in the presence of …

Laczya, Label

30. How does lactose induce the Laczya operon

Lactose, Laczya

31. Another important mechanism that governs the level of Laczya transcription in E

Level, Laczya

32. DE(Laczya)514 leaves lacI intact, removes the lac promoter, lacZ, lacY, and lacA entirely, and leaves intact the terminator for the downstream, oppositely oriented, cynX (Fig

Laczya, Leaves, Laci, Lac, Lacz, Lacy, Laca

33. Coli is grown on a mix of carbon sources, such as glucose and lactose, it will first consume the glucose and then induce expression of Laczya to consume the lactose

Lactose, Laczya

34. Laczya on RegulonDB; lac operon on Wikipedia; References

Laczya, Lac

35. In contrast, at 26°C, expression of orf6-Laczya, sycB-Laczya, ysaE-Laczya, ysrS-Laczya, and rcsB-Laczya increased early during the logarithmic phase of bacterial growth, and then expression dropped rapidly to baseline levels as the cultures of these strains entered into the stationary phase of …

Laczya, Logarithmic, Levels

36. In this work, an in-frame deletion in invF was constructed and tested for the expression of Φ( sigD-Laczya ), sipC ::Tn 5lacZY , and a plasmid-encoded Φ( sicA-Laczya )

Laczya

37. Actose metabolic activity in the lac operon is represented in the image below bd Determine the nature of the expression of the Laczya constihutive, inducible, or no transcription in the presence of represented by L and label e each genotype as Assume that all promoters are wild type laci mutant cannot bind to the operator 卢 o ↓laci mutant always brds to the operator Operator mutant thatl

Lac, Laczya, Label, Laci

38. Other Transcription-Units in the Same Operon: lacYA (lacYp), Laczya (lacZp4), Laczya (lacZp2), Laczya (lacZp3) Summary: The lac operon is controlled by four promoters, P1, which is the principal promoter, P2, and P3 and P4, the weaker promoters [Xiong91, Eschenlauer91]

Lacya, Lacyp, Laczya, Lac

39. Other Transcription-Units in the Same Operon: lacYA (lacYp), Laczya (lacZp4), Laczya (lacZp3), Laczya (lacZp1) Summary: The lac operon is controlled by four promoters, P1, which is the principal promoter, P2, and P3 and P4, the weaker promoters [Xiong91, Eschenlauer91]

Lacya, Lacyp, Laczya, Lac

40. Three different mini-Tn5 plasmids, containing a tetracycline-inducible promoter, P tet and a regulatory gene, tetR, in operon fusions with a reporter gene system (Laczya, luxCDABE or gfp), were constructed.These biosensor constructs responded to low levels of tetracyclines by producing β-galactosidase, light or green fluorescent protein.

Laczya, Luxcdabe, Low, Levels, Light

41. The mutations that the DH5-Alpha strain has are: dlacZ Delta M15 Delta(Laczya-argF) U169 recA1 endA1 hsdR17(rK-mK+) supE44 thi-1 gyrA96 relA1 (2)

Laczya

42. For example, DE(Laczya)514::cat and DE(Laczya)514::kan refer to mutations made with the same primers and pKD3 (cat) or pKD4 (kan) as template, respectively

Laczya

43. After eviction of the resistance gene, the mutation(s) is simply called DE(Laczya)514, as …

Laczya

44. A popA-Laczya reporter strain of RS1002, RK10001, was constructed using the pK18mobsacB-based plasmid ppop3 (Yoshimochi et al., 2009b)

Laczya

45. F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(Laczya-argF)U169, hsdR17(rK- mK+), λ– An Hoffman-Berling 1100 strain derivative (Meselson68) Promega also lists phoA

Laczya, Lists

46. The Laczya site includes binding sites for CRP, LacI and RNAP

Laczya, Laci

47. ラクトースオペロンはLaczyaともいわれるが、これはラクトースオペロンがラクトース代謝系の3つの構造遺伝子lacZ、lacY、lacAから構成されているためである。 lacZ:β-ガラクトシダーゼ beta-galactosidase (EC 3.2.1.23, 反応)(LacZ)をコードする遺伝子である。

Laczya, Lacz, Lacy, Laca

48. A tapV‐Laczya reporter fusion was generated and integrated into chromosome of OE1‐1 (the wild‐type strain) with the Tn7‐based chromosomal integration system to generate the reporter strain RQC0341

Laczya

49. Note that integration of tapV‐Laczya did not affect …

Laczya

50. Sion analysis involving Laczya and fdxN or ORFU3 provided evidence that the putative UAS is responsible for regulation of both ORFUl-/<*tC-/<±dv"and ORFU2-ORFU3 operons in opposite orientations, and that the control of the latter is stricter than that of the former.

Laczya, Lt, Latter

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