Use Counterstains In A Sentence

Counterstains

[ˈkoun(t)ərˌstān]

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Looking for sentences with "Counterstains"? Here are some examples.

1. Use counterstains when appropriate, as in the following examples When titering CD8 mAbs, stain each sample with constant amount of a CD3 antibody, usually labeled with a fluorophore that doesn’t interact with the label on the antibody you are titering. 6.
2. Counterstaining aids in visualization and target localization, facilitating interpretation of morphology and cell structure within the tissue section. Our nuclear counterstains are packaged as convenient, ready-to-use solutions for use on individual slides or in staining dishes.
3. Example of the use of special stains and counterstains. Prussian blue i ron stain kit staining iron (blue) in liver tissue. Nuclei and background were counterstained with nuclear fast red. Chromogenic counterstains Chromogenic counterstains are used when the primary antibody is visualized using HRP or alkaline phospatase combined with DAB, AEC, or similar enzyme substrates.
4. counterstains stain cell nuclei and are packaged as convenient, ready-to-use solutions for use on slides or in staining dishes. Available to you (see selection guide below): Vector ® Hematoxylin — based on Gill's formulation, can be adjusted for optimal intensity; suitable for use with non-aqueous and aqueous mounting media and is mercury
5. Where counterstains come in As you probably know, most cells are. When it comes to immunohistochemistry (IHC) we spend a big chunk of time figuring out which antibody to use and which concentrations to try, but how many of. When it comes to immunohistochemistry (IHC) we spend a big chunk of time figuring out which antibody to use and which
6. Haematoxylin counterstains have the advantage over others in that they provide a good picture of the morphology of the nucleus as well, often allowing identification of specific cell types. Cite 2
7. counterstains provide contrast as well as knowledge about localization within the tissue sample (for instance by visualizing nuclei or filamentous actin). What to consider when selecting counterstains? Ensure that the counterstain can be easily spectrally distinguished from your precipitate color or fluorescent label.
8. However, for counterstaining this may not be the best stain to use, since a counterstain should be a different colour to the main technique so that it does not confuse identifi-cation. The blue/purple colours of haematoxylin often overlap with the colour of the main technique, so other nuclear counterstains are needed.
9. The counterstains described in this section are compatible with a wide range of cytological labeling techniques, including direct or indirect antibody-based detection methods, in situ hybridization and the detection of specific subcellular structures with fluorescent probes such as the mitochondrion-selective MitoTracker reagents (Probes for
10. Use counterstains when appropriate, as in the following examples When titering CD8 mAbs, stain each sample with constant amount of a CD3 antibody, usually labeled with a fluorophore that doesn’t interact with the label on the antibody you are titering. When titering an anti-HLA-DR mAb, you might use as counterstains three antibodies:
11. These counterstains are used for the Fast Red chromogens: methyl green, brilliant green and soluble hematoxylin. counterstains. After staining the target antigen by IHC, a secondary stain is usually applied to provide contrast that helps the primary stain more distinct. While many of these stains show specificity for discrete antigens or
12. counterstains for Fluorescent Immunostaining Systems Common counterstaining options for fluorescent immunostaining involve the use of fluorescent chemicals such as DAPI or Hoechst . DAPI (4', 6-diamidino-2-phenylindole) and Hoechst 33342 are common nuclear dyes that work by intercalating into the DNA and producing a strong blue color under UV
13. Although they may not stain specific cell components, you still can use them to enhance the contrast as counterstains. Since most biological samples are watery tissues (water is accounting for 70% of the weight of our cells), water-based pigments will work better. Oil paints or acrylic paints may not be compatible.

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