Use Counterstaining In A Sentence

Counterstaining

[ˈkoun(t)ərˌstān]

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Looking for sentences with "Counterstaining"? Here are some examples.

1. Eosin and Cytoplasmic counterstaining. Clearly, hematoxylin and Nuclear Fast Red stain, for all intents and purposes, just the nucleus. What if we have a nuclear signal and we want to stain the cytoplasm? In this case, use eosin. Interestingly, the base compound of eosin is fluorescein (Figure 4-43). Specifically, it results from the action of
2. Suggested Use. counterstaining for immunohistochemistry or other special stains. Staining Procedure. Sections to distilled water after IHC. Stain in methyl green solution for 5 minutes at room temperature (60 C may produce slightly stronger stain). Rinse in distilled water (sections will look blue). 6.
3. counterstaining aids in visualization and target localization, facilitating interpretation of morphology and cell structure within the tissue section. Our nuclear counterstains are packaged as convenient, ready-to-use solutions for use on individual slides or in staining dishes.
4. When it comes to immunohistochemistry (IHC) we spend a big chunk of time figuring out which antibody to use and which concentrations to try, but how many of us take the time to think about which counterstain would be the best choice for our tissue and the message we want to portray? Believe it or not, counterstaining can have a big impact on the final result.
5. Immunofluorescence Staining and counterstaining. In addition to immunofluorescence, cells are typically counterstained with DNA or cytoskeleton-binding dyes to help identify cellular organelles and structures. Fluorescent microscopy is used for individual cell identification and analysis.
6. Important Considerations: When choosing counterstaining options in ICC/IF, it is important to ensure that the chosen counterstain and fluorochromes in use for the target proteins are displaying a negligible spectral overlap. This will allow for an easy distinguishing of signals and in eliminating the bleedthrough effect.
7. Suggested Use . counterstaining for immunohistochemistry or other special stains. Staining Procedure . 1. Sections to distilled water after IHC. 2. Stain in methyl green solution for 5 minutes at room temperature (60 C may produce slightly stronger stain). 3. Rinse in distilled water (sections will look blue). 4.
8. Counterstaining. Use a counterstain for specific morphologies or structures to aid localization of your primary antibody. When performing IHC, it is important to use a counterstain, so that you can see where the staining from the antibody is in relation to the cellular structures within the tissue.
9. Suggested Use. counterstaining for immunohistochemistry or other special stains. Staining Procedure. Sections to distilled water after IHC. Stain in methyl green solution for 5 minutes at room temperature (60 C may produce slightly stronger stain). Rinse in distilled water (sections will look blue).
10. Immunohistochemistry (IHC) is a detail-oriented procedure. That point has been alluded to through previous blog entries and indeed, in the last entry I likened the application to cooking and requiring some artistic finesse to achieve an outstanding final result. In a similar manner, counterstaining, like barbeque sauce (my apologies to vegetarian readers), is applied, oftentimes dogmatically
11. Use the fixation protocol appropriate for your sample. DAPI staining is normally performed after all other staining. Note that fixation and permeabilization of the sample are not necessary for counterstaining with DAPI. counterstaining protocol. Equilibrate the sample briefly with phosphate-buffered saline (PBS).

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