Use Counterstain In A Sentence

Counterstain

[ˈkoun(t)ərˌstān]

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Looking for sentences with "Counterstain"? Here are some examples.

1. A counterstain introduces color to specific cellular structures to provide contrast to the colored enzyme substrate. Counterstaining aids in visualization and target localization, facilitating interpretation of morphology and cell structure within the tissue section. Our nuclear counterstains are packaged as convenient, ready-to-use solutions
2. counterstain definition, a second stain of a different color applied to a microscopic specimen and used to color and contrast those parts not retaining the first stain. See more.
3. A counterstain is a stain with colour contrasting to the principal stain, making the stained structure easily visible using a microscope.. Examples include the malachite green counterstain to the fuchsine stain in the Gimenez staining technique and the eosin counterstain to haematoxylin in the H&E stain. In Gram staining, crystal violet stains only Gram-positive bacteria, and safranin
4. 1. A counterstain is a stain with colour contrasting to the principal stain, making the stained structure easily visible using a microscope . Examples include the malachite green counterstain to the fuchsine stain in the Gimenez staining technique and the eosin counterstain to haematoxylin in the H&E stain. In Gram staining, crystal violet stains only : 2.
5. Of course, you cannot use the blue color of the hematoxylin with NBT/BCIP, since the precipitate is the same color blue. You need to use Nuclear Fast Red as the nuclear counterstain if using NBT/BCIP as the chromogen. Since hematoxylin basically stains only the nuclei, it can be used at maximum intensity if the signal is cytoplasmic based.
6. Safranin it is referred as counterstain. Procedures of Gram staining. Prepare a smear of bacteria culture and heat fix the smear. Smear are first stained with Crystal Violet which is a basic dye it imparts purple color to all cells. Washed the slide with distilled water for seconds.
7. Hematoxylin, which is usually used together with eosin (H&E stain), is one of the most commonly used dyes in diagnostic histology.It is also used alone as a nuclear counterstain in IHC. Oxidized hematoxylin is combined with aluminum ions to form an active metal–dye complex that stains the nuclei of mammalian cells blue by binding to lysine residues on nuclear histones.
8. One other circumstance where the use of a counterstain may be questionable would be in a multiple antigen labeling assay. In double and triple antigen staining on the same section, there may be abundant color of specifically stained cells and structures. The addition of a third or fourth color on these sections by way of a counterstain may
9. Safranin (also Safranin O or basic red 2) a biological stain used in histology and cytology Use: a) as a counterstain in some staining protocols, colouring all cell nuclei red. b) This is the classic counterstain in both Gram stains, and endospore staining. c) It can also be used for the detection of cartilage, mucin and mast cell granules
10. The counterstain cannot be absorbed and the components will retain the color of the primary stain is the primary stain is not washed out. If primary stain is removed, the decolorizing cellular components will accept and assume the contrasting color of the counterstain.
11. Ensure that the counterstain can be easily spectrally distinguished from your precipitate color or fluorescent label. 5. Use counterstains when appropriate, as in the following examples When titering CD8 mAbs, stain each sample with constant amount of a CD3 antibody, usually labeled with a fluorophore that doesn’t interact with the label on
12. Important Considerations: When choosing counterstaining options in ICC/IF, it is important to ensure that the chosen counterstain and fluorochromes in use for the target proteins are displaying a negligible spectral overlap. This will allow for an easy distinguishing of signals and in eliminating the bleedthrough effect.
13. Counterstain/Substrate Compatibility Table This table is designed as a reference to determine the optimal counterstain/substrate combination for your application. Considerations should be given to tissue type, antigen unmasking protocol and other detection parameters to achieve the desired staining intensity.
14. counterstain definition is - to stain (something, such as a microscopy specimen) so as to color parts (such as the cytoplasm of cells) not colored by another stain (such as a nuclear stain).
15. For use: Transfer a small amount of the reagent to a dispensing container that can be closed when not in use. counterstain . Safranin and dilute carbol-fuchsin are commonly used counterstain in Gram staining procedure, another being Neutral red (it stains gonococci and meningococci well) Preparation of Safranin
16. Ensure that the counterstain can be easily spectrally distinguished from your precipitate color or fluorescent label. For example, when using red emitting fluorophores, select a blue nuclear counterstain such as DAPI or Hoechst 33342 (Tables 4 and 5) To save time, consider using pre-made mounting media already containing counterstains
17. Capsule stain is a type of differential stain which involves the use of two stains; primary stain and the counterstain. Here, it's worth noting that for most part, capsules are non-ionic. As a result, acidic and basic stains will often fail to adhere on to them.
18. Use a counterstain for specific morphologies or structures to aid localization of your primary antibody. When performing IHC, it is important to use a counterstain, so that you can see where the staining from the antibody is in relation to the cellular structures within the tissue.
19. 7.counterstain with Safarin (1-2 mins) 8.Wash off with distilled water 9. Blot Dry 10. Examine 100x immersion oil. While staining a mixed culture of Gram-positive and Gram-negative bacteria, switching the order of steps 2 and 3 would result in _____. All Bacteria will appear pink since the iodine mordant will not form an insoluble complex by

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